Ordering tryptophan synthase genes of Pseudomonas aeruginosa by cloning in Escherichia coli

Structure and regulation of the anthranilate synthase genes in Pseudomonas aeruginosa: II. Cloning and expression in Escherichia coli.

The genes for the large and small subunits of anthranilate synthase (trpE and trpG, respectively) have been cloned from Pseudomonas aeruginosa PAC174 into E. coli by R-prime formation with the broad-host-range plasmid R68.44. Sequential subcloning into plasmid vectors reduced the active Pseudomonas DNA fragment to a length of 3.1 kb. We obtained evidence that this region contains the promoter f...

cloning of arginine deiminase from pseudomonas aeruginosa in escherichia coli

optimization of the expression of genes encoding poly (3-hydroxyalkanoate) synthase from pseudomonas aeruginosa ptcc 1310 in escherichia coli

over the years, the use of plastics has complicated the problem of disposal of solid wastes. one strategy to reduce plastic waste is the use of biodegradable plastics. a group of these plastics are polyhydroxyalkanoates (phas). to date more than 250 different microorganisms are known to synthesize and accumulate pha. most pseu-domonas strains are able to accumulate mcl-pha. in previous studies,...

Nucleotide sequence of the genes for tryptophan synthase in Pseudomonas aeruginosa.

We have determined the DNA sequence of the two adjacent genes for the alpha and beta chains of tryptophan synthase in Pseudomonas aeruginosa, along with 34 5'-flanking and 799 3'-flanking base pairs. The gene order is trpBA as predicted from earlier genetic studies, and the two cistrons overlap by 4 bp; a ribosome binding site for the second gene is evident in the coding sequence of the first g...

Construction of New Genetic Tools as Alternatives for Protein Overexpression in Escherichia coli and Pseudomonas aeruginosa

Background: Pseudomonas protein expression in E. coli is known to be a setback due to signifi cant genetic variation and absence of several genetic elements in E. coli for regulation and activation of Pseudomonas proteins. Modifi cations in promoter/repressor system and shuttle plasmid maintenance have made the expression of stable and active Pseudomonas protein possible in bot...